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KMID : 1025520030450060911
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Journal of Animal Science and Technology
2003 Volume.45 No. 6 p.911 ~ p.916
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Genetic Variation of Mitochondrial DNA in Duroc (Sus Scrofa) Using Single Stranded Conformation Polymorphism Analysis
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조인철/Cho, I. C.
정용환/정진관/성필남/김병우/이정규/전진태/Jung, Y. H./Jung, J. K./Seung, P. N./Kim, B. W./Lee, J. G./Jeon, J. T.
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Abstract
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The mitochondrial DNA(mtDNA) D-loop region was amplified from Duroc(Su scrofa) by polymerase chain reaction(PCR). The oligonucleotide primer used to amplify the Sus scrofa mtDNA D-loop region was designed using tRNA-Pro and tRNA-Phe sequence in mtDNA regions highly conserved in many other amimal species. There were 1,145 base paris(bp) in the D-loop region. the middle of the region contained 10 tandem repeat of an 10-bp Sus scrofa-specifie sequence, TACACGTGCG. We designed primers for PCR-mediated single stranded conformation polymorphism(SSCP) analysis that amplified of denatured amplification products was carried out by polyacrylamide(8％) gel electrophoresis followed by ethidium bromide staining. The SSCP analysis identified two band patterns(A and B) and comparision of these two nucleotide sequences identified 21 base substitutions. These results show that SSCP analysis of the D-loop region is useful for detecting the genetic polvmorphism.
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